Changes in antibiotic resistance genes and mobile genetic elements during cattle manure composting after inoculation with Bacillus subtilis. (November 2019)

• Record Type: Journal Article
• Authors: Duan, Manli; Zhang, Yuhua; Zhou, Beibei; Wang, Quanjiu; Gu, Jie; Liu, Guohuan; Qin, Zhenlun; Li, Zhijian
• Is Part Of: Bioresource technology. Volume 292(2019)
• Publication Date: 2019-11
• Subjects: Antibiotic resistance gene -- Bacillus subtilis -- Bacterial community -- Composting -- intI1; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2019.122011
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: sul1 mainly be responsible for changes in ARGs during composting. Adding Bacillus subtilis at 0.5% reduced relative abundances of ARGs and MGEs. Pathogenic bacteria were largely eliminated in compost with Bacillus subtilis . intI1, Firmicutes, and pH were the main drivers of variations in ARGs. Abstract: This study explored the effects of Bacillus subtilis at four levels (0, 0.5%, 1%, and 2% w/w compost) on the variations in ARGs, mobile genetic elements (MGEs), and the bacterial community during composting. The composting process had a greater impact on ARGs than Bacillus subtilis . The main ARG detected was sul1 . The addition of Bacillus subtilis at 0.5% reduced the relative abundances of ARGs, MGEs, and human pathogenic bacteria (by 2–3 logs) in the mature products. Network and redundancy analyses suggested that intI1, Firmicutes, and pH were mainly responsible for the changes in ARGs, thus controlling these factors might help to inhibit the spread of ARGs.