Polyhydroxyalkanoates (PHA) production from fermented thermal-hydrolyzed sludge by PHA-storing denitrifiers integrating PHA accumulation with nitrate removal. (November 2019)

• Record Type: Journal Article
• Authors: Tu, Weiming; Zhang, Dandan; Wang, Hui; Lin, Ziyu
• Is Part Of: Bioresource technology. Volume 292(2019)
• Publication Date: 2019-11
• Subjects: AFD aerobic feeding and discharge -- CDW cell dry weight -- COD chemical oxygen demand -- COD/N/P chemical oxygen demand to nitrogen and phosphorus ratio -- DO dissolved oxygen -- HRT hydraulic retention time -- MMCs mixed microbial cultures -- NH4-N ammonia nitrogen -- NO nitric oxide -- NO2-N nitrite -- NO3-N nitrate, N2O, nitrous oxide -- PHA polyhydroxyalkanoates -- PHB polyhydroxybutyrate -- PHV polyhydroxyvalerate -- qPHA PHA production rate -- qVFA VFA uptake rate -- SBR sequencing batch reactor -- SCOD soluble chemical oxygen demand -- sbCOD slowly biodegradable COD -- SRT sludge retention time -- TN total nitrogen -- TP total phosphorus -- VFA volatile fatty acids -- WWTP wastewater treatment plant -- X active biomass -- YPHA/VFA PHA storage yield on VFA -- YX/S growth yield on substrates; Thermal-hydrolyzed sludge -- Polyhydroxyalkanoates -- Mixed microbial culture -- Denitrification; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2019.121895
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphic abstract: Highlights: Aerobic-feast and anoxic-famine regime was applied for culture selection. Energy input of aeration was reduced by 79% compared with the aerobic regime. PHA yield on VFA of 0.47 gCOD/gCOD and nitrate removal of 98% were achieved. Accumulation of nitrite and nitrous oxide during the process were investigated. Integration of PHA production with wastewater treatment was designed. Abstract: Polyhydroxyalkanoates (PHA) production from fermented thermal-hydrolyzed sludge was conducted by mixed microbial cultures (MMCs) in the study. An MMC enriched in the species Brachymonas_denitrificans (60.18%) was selected under an aerobic feast/famine regime, which is capable of denitrification and accumulating PHA. To take advantage of the PHA-storing denitrifiers, an aerobic-feast/anoxic-famine regime was applied to integrate culture selection with denitrification. The results showed that cultures enriched under the regime exhibited a PHA storage capacity with PHA yield on VFA of 0.47 gCOD/gCOD and well denitrification performance achieving nitrate removal of 98%. Moreover, the aerobic-feast/anoxic-famine regime could originate a comparable maximum PHA content to the complete aerobic feast/famine regime (49.7 wt% versus. 47.1 wt%, respectively), yet reduce aeration energy input by 79% in the culture selection process. Finally, this study investigated the accumulation of nitrite and nitrous oxide during PHA based denitrification and the feasibility of integrating the process with wastewater treatment.