Effect of hexavalent chromium on the biodegradation of tetrabromobisphenol A (TBBPA) by Pycnoporus sanguineus. (November 2019)

• Record Type: Journal Article
• Authors: Feng, Mi; Li, Haixiang; You, Shaohong; Zhang, Jun; Lin, Hua; Wang, Meiqian; Zhou, Jiahua
• Is Part Of: Chemosphere. Volume 235(2019)
• Page Start: 995
• Page End: 1006
• Publication Date: 2019-11
• Subjects: Pycnoporus sanguineus -- Hexavalent chromium -- Tetrabromobisphenol A -- Co-contamination -- Biodegradation; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2019.07.025
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: The influence of Cr(VI) on the degradation of tetrabromobisphenol A (TBBPA) by a typical species of white rot fungi, Pycnoporus sanguineus, was investigated in this study. The results showed that P. sanguineus together with its intracellular and extracellular enzyme could effectively degrade TBBPA. The degradation efficiency of TBBPA by both P. sanguineus and its enzymes decreased significantly when Cr(VI) concentration increased from 0 to 40 mg/L. The subsequent analysis about cellular distribution of TBBPA showed that the extracellular amount of TBBPA increased with the increment of Cr(VI) concentration, but the content of TBBPA inside fungal cells exhibited an opposite variation tendency. The inhibition of TBBPA degradation by P. sanguineus was partly attributed to the increase of cell membrane permeability and the decrease of cell membrane fluidity caused by Cr(VI). In addition, the decline of H + -ATPase and Mg 2+ -ATPase activities was also an important factor contributing to the suppression of TBBPA degradation in the system containing concomitant Cr(VI). Moreover, the activities of two typical extracellular lignin-degrading enzymes of P. sanguineus, MnP and Lac, were found to descend with ascended Cr(VI) level. Cr(VI) could also obviously suppress the gene expression of four intracellular enzymes implicated in TBBPA degradation, including two cytochrome P450s, glutathione S-transferases and pentachlorophenol 4-monooxygenase, which resulted in a decline of TBBPA degradation efficiency by fungal cells and intracellular enzyme in the presence of Cr(VI). Overall, this study provides new insights into the characteristics and mechanisms involved in TBBPA biodegradation by white rot fungi in an environment where heavy metals co-exist. Graphical abstract: Image 1 Highlights: Cr(VI) inhibited the biodegradation of TBBPA by P. sanguineus and its enzymes. The amount of TBBPA inside or outside the cell varied with various level of Cr(VI). Cr(VI) caused changes of cell membrane characteristics and inhibited ATPase. TBBPA degradation was inhibited due to the suppression of MnP and Lac by Cr(VI). Cr(VI) inhibited expression of intracellular enzymes involved in TBBPA degradation.