The influence of temperature and salinity on the impacts of lead in Mytilus galloprovincialis. (November 2019)

• Record Type: Journal Article
• Authors: Freitas, Rosa; Leite, Carla; Pinto, João; Costa, Marcelo; Monteiro, Rui; Henriques, Bruno; Di Martino, Francesco; Coppola, Francesca; Soares, Amadeu M.V.M.; Solé, Montserrat; Pereira, Eduarda
• Is Part Of: Chemosphere. Volume 235(2019)
• Page Start: 403
• Page End: 412
• Publication Date: 2019-11
• Subjects: Climate change -- metal(oid)s -- Bioaccumulation -- Mussels -- Oxidative stress -- Metabolism; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2019.05.221
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Mussels, such as the marine bivalve Mytilus galloprovincialis are sentinels for marine pollution but they are also excellent bioindicators under laboratory conditions. For that, in this study we tested the modulation of biochemical responses under realistic concentrations of the toxic metal Lead (Pb) in water for 28 days under different conditions of salinity and temperature, including control condition (temperature 17 ± 1.0 °C and salinity 30 ± 1.0) as well as those within the range expected to occur due to climate change predictions (± 5 in salinity and + 4 °C in temperature). A comprehensive set of biomarkers was applied to search on modulation of biochemical responses in terms of energy metabolism, energy reserves, oxidative stress and damage occurrence in lipids, proteins as well as neurotoxicity signs. The application of an integrative Principal Coordinates Ordination (PCO) tool was successful and demonstrated that Pb caused an increase in the detoxification activity mainly evidenced by glutathione S-transferases and that the salinities 25 and 35 were, even in un-exposed mussels, responsible for cell damage seen as increased levels of lipid peroxidation (at salinity 25) and oxidised proteins (at salinity 35). Highlights: Metabolism increased at lower salinity and higher temperature in Pb exposed mussels. Overall, exposure to Pb increased detoxification activity measured as GSTs. Antioxidant defences failed to prevent LPO at the lowest salinity in controls. Damaged proteins occurred at the highest salinity in unexposed mussels.