Consolidated bioprocessing performance of bacterial consortium EMSD5 on hemicellulose for isopropanol production. (November 2019)

• Record Type: Journal Article
• Authors: Liu, Liang; Yang, Jinshui; Yang, Yi; Luo, Lijin; Wang, Ruonan; Zhang, Yu; Yuan, Hongli
• Is Part Of: Bioresource technology. Volume 292(2019)
• Publication Date: 2019-11
• Subjects: Consolidated bioprocessing -- Isopropanol -- EMSD5 -- Corncob -- Xylan structure; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2019.121965
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Bacterial consortium EMSD5 produced isopropanol from xylans efficiently. 7.00 g/L isopropanol was produced from unpretreated corncob by consortia-based CBP. Arabinose residues of xylan could enhance the CBP performance. Clostridium beijerinckii and lignocellulose degraders performed CBP synergistically. Abstract: Consolidated bioprocessing (CBP) of lignocellulose by bacterial consortium for isopropanol production is considered as the most promising strategy. However, low utilization of xylan caused by the complex sidechain structure remains inhibit the conversion of full-biomass. In this study, isopropanol production from different lignocelluloses by the consortium EMSD5 through CBP was performed. A total of 7.00 g/L of isopropanol was obtained from corncob by optimizing fermentation conditions. Isopropanol production by EMSD5 was mainly based on utilizing xylan in corncob and isopropanol titer was increased by 47.71% and reached up to 8.39 g/L using arabinoxylan compared with linear xylan. The analysis of community structures and the isolation of key strain confirmed the enrichment of the isopropanol producer, Clostridium beijierinckii, in the bacterial community when it was incubated with corn glucuronoarabinoxylan and the cooperation between C. beijerinckii and lignocellulose degraders. The unique features of EMSD5 could lead to large-scale isopropanol production using lignocellulose.