Amelogenin domain‐containing NlChP38 is necessary for normal ovulation in the brown planthopper. Issue 5 (4th March 2019)

• Record Type: Journal Article
• Authors: Lou, Y.‐H.; Lu, J.‐B.; Li, D.‐T.; Ye, Y.‐X.; Luo, X.‐M.; Zhang, C.‐X.
• Is Part Of: Insect molecular biology. Volume 28:Issue 5(2019)
• Page Start: 605
• Page End: 615
• Publication Date: 2019-03-04
• Subjects: brown planthopper -- eggshell -- eggshell protein -- ovulation -- amelogenin domain; Insects -- Molecular aspects -- Periodicals; 595.7
• DOI: 10.1111/imb.12576
• Languages: English
• ISSNs: 0962-1075

Abstract

Abstract: The brown planthopper (BPH), Nilaparvata lugens, is a major threat to rice production. The eggshell plays an important role in insect reproduction. The constituents and formation process of BPH eggshells remains largely unknown. Here, we report a novel eggshell‐associated protein, NlChP38, containing an amelogenin domain, that is essential for normal ovulation in the BPH. NlChP38 is specifically expressed in the follicular cells from egg chambers at both RNA and protein levels. RNA interference of NlChP38 resulted in oocytes with loose and thin eggshell structure and caused ovulation difficulties. Immunofluorescence localization showed NlChP38 is deposited between follicular cells and oocytes during late choriogenesis. These results indicate that NlChP38 plays an important role in eggshell formation and could be a potential target for RNA interference control of the BPH.