Rabies Virus 31D Peptide‐[P(VP‐co‐AA)] Conjugates: Synthesis, Characterization and Cytotoxicity Evaluation. Issue 32 (28th August 2019)

• Record Type: Journal Article
• Authors: Arayici, Pelin Pelit; Acar, Tayfun; Ucar, Burcu; Karahan, Mesut; Arslan, Belkis Atasever; Mustafaeva, Zeynep
• Is Part Of: ChemistrySelect. Volume 4:Issue 32(2019)
• Page Start: 9483
• Page End: 9487
• Publication Date: 2019-08-28
• Subjects: Conjugation -- Cytotoxicity -- Polymeric Adjuvant -- Rabies -- SPPS; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/slct.201901375
• Languages: English
• ISSNs: 2365-6549

Abstract

Abstract: The prevention of rabies disease, which has been the subject of many pieces of researches since ancient times, has become possible by vaccination. Therefore, new generation vaccine systems should be developed to achieve more effective, accessible and reliable vaccines than conventional vaccines against this disease. In this study, modificated Rabies virus 31D antigenic peptide epitope was synthesized by microwave supported solid phase peptide synthesis method and the synthesized peptide was characterized. Bioconjugates of the antigenic peptide epitope with poly (N‐Vinyl‐2‐pyrrolidone‐co‐acrylic acid) that a biocompatible polymer was synthesized synthetically were synthesized at different ratios in the presence of 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide. Characterization of bioconjugates was performed with Gel Permeation Chromatography and ZetaSizer. Cytotoxicity test was carried out using ECV304 human epithelial cells to determine the biocompatibility of peptide, polymer and bioconjugates. It is thought that the produced biocompatible synthetic peptide‐polymer based rabies vaccine systems will contribute greatly to the vaccination studies. Abstract : 31D peptide from antigenic peptide epitopes of rabies disease was synthesized in high purity by minor modifications and bioconjugates were successfully synthesized in the presence of N‐(3‐Dimethylaminopropyl)‐N′‐ethylcarbodiimide with a biocompatible polymer [P(VP‐co‐AA)]. These bioconjugates have been shown to be biocompatible and results obtained suggest that the synthesized bioconjugates will be an important model in vaccine studies.