One-step sensitive thrombin detection based on a nanofibrous sensing platform. Issue 34 (6th August 2019)

• Record Type: Journal Article
• Authors: Li, Xiaoling; Wu, Yuyang; Niu, Jingjing; Jiang, Dagang; Xiao, Dan; Zhou, Cuisong
• Is Part Of: Journal of materials chemistry. Volume 7:Issue 34(2019)
• Page Start: 5161
• Page End: 5169
• Publication Date: 2019-08-06
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/c9tb01098j
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : By integration of a new PiDSD process, CHA amplification and ThT binding, one-step fluorescent detection of thrombin can be achieved by immersing a porous and 3-direction structural B-H2-functionalized nanofibrous membrane in a testing solution. Abstract : Convenient and time-saving one-step strategies for detecting ultralow concentrations of protein biomarkers play key roles in rapid disease diagnosis. In this study, we report a one-step detection method based on a nanofibrous sensing platform via the combination of proximity-induced DNA strand displacement (PiDSD), catalytic hairpin assembly (CHA) amplification and thioflavin T (ThT) binding. The interface behaviors on the nanofibrous membrane were studied to promote interface reaction kinetics and thermodynamics. Thrombin was used as a model biomarker, and the nanofibrous sensing platform achieved a limit of detection as low as 1.0 pM, a wide linear range of 50 pM to 5 nM, excellent specificity and good long-term stability. Compared with previous one-step thrombin detection methods, our one-step detection method is label-free, convenient and much more sensitive; it has potential applications for protein detection in point-to-care testing (POCT) and early diagnosis.