Identification of a novel turn-on albumin binding small-molecule bioprobe in live zebrafish and its potential application in drug discovery. (December 2019) - Chun, Hang-Suk
Abstract: A novel small-molecule fluorescent probe (4f ) for staining blood in live zebrafish was identified. Compound4f turns on the fluorescent property in the presence of albumin in the blood and it mainly binds on the site II of human serum albumin.4f efficiently visualized the blood vessels in live zebrafish, Hence, it could be used as an alternative blood vessel imaging tool. Compound4f applied to test for anti-cancer drug screening in live zebrafish, and showed a potential as an effective in vivo drug screening agent. Highlights: A novel small-molecule fluorescent bioprobe (4f ) for staining blood was identified in live zebrafish. 4f turns on the fluorescent property in the presence of albumin in the blood and mainly binds on the site II of HSA. 4f showed a potential as an effective in vivo drug screening agent from the test of anti-cancer drug screening in live zebrafish.