Carrageenan-based functional hydrogel film reinforced with sulfur nanoparticles and grapefruit seed extract for wound healing application. (15th November 2019)

• Record Type: Journal Article
• Authors: Jaiswal, Lily; Shankar, Shiv; Rhim, Jong-Whan
• Is Part Of: Carbohydrate polymers. Volume 224(2019)
• Publication Date: 2019-11-15
• Subjects: κ-Carrageenan -- Hydrogel film -- Sulfur nanoparticles -- Grapefruit seed extract -- Antibacterial activity -- Wound healing; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2019.115191
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: Carrageenan-based wound dressing was prepared with SNP and GSE. In vivo wound healing effect was tested using wounded Sprague-Dawley rats. Mechanical strength, water swelling, and UV-barrier properties were improved. The films exhibited strong antibacterial activity against S. epidermis and E. coli . Carr/GSE/SNP3% film showed an excellent wound healing effect. Abstract: κ-Carrageenan-based (Carr) functional wound healing hydrogel films were prepared by incorporating chitosan capped sulfur nanoparticles (SNP) and grapefruit seed extract (GSE). In vivo wound healing effect of the hydrogel films was tested using artificially wounded male Sprague-Dawley rats. The hydrogel film (Carr/GSE/SNP) showed higher mechanical strength, swelling ratio and ultraviolet barrier properties than the carrageenan film, but the water vapor permeability and water solubility were decreased. The hydrogel film showed antibacterial activity strong enough to destroy Staphylococcus epidermis and Escherichia coli within 3 h of incubation and showed high biocompatibility against mouse fibroblast (L929 cell lines). The hydrogel film (Carr/GSE/SNP3%) showed excellent wound healing effect (1.3% wound area after 2 weeks) compared to the control group (31% wound area after 2 weeks) through in vivo wound healing test. Histological examination showed the complete appearance of the healed epidermis. The Carr/GSE/SNP hydrogel films are most likely to be used for the treatment of full-thickness wounds.