Adenosine stimulates neuromedin U mRNA expression in the rat pars tuberalis. (1st October 2019)

• Record Type: Journal Article
• Authors: Aizawa, Sayaka; Gu, Tingting; Kaminoda, Arisa; Fujioka, Ryuya; Ojima, Fumiya; Sakata, Ichiro; Sakai, Takafumi; Ogoshi, Maho; Takahashi, Sumio; Takeuchi, Sakae
• Is Part Of: Molecular and cellular endocrinology. Volume 496(2019)
• Publication Date: 2019-10-01
• Subjects: Neuromedin U -- Pars tuberalis -- Adenosine receptor -- cAMP response element -- Circadian rhythm -- Melatonin; Endocrinology -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Endocrinology -- Periodicals; Hormones -- Periodicals; Endocrinologie -- Périodiques; Cytology; Endocrinology; Molecular biology; Periodicals; 573.4
• DOI: 10.1016/j.mce.2019.110518
• Languages: English
• ISSNs: 0303-7207

Abstract

Abstract: Neuromedin U (NMU) shows circadian expression in the rat pars tuberalis (PT), and is known to be suppressed by melatonin. Here we examined the involvement of adenosine in the regulation of Nmu expression. We found that the rat PT expressed adenosine receptor A2b and that an adenosine receptor agonist, NECA, stimulated Nmu expression in brain slice cultures. In vitro promoter assays revealed that NECA stimulated Nmu promoter activity via a cAMP response element (CRE) in the presence of adenosine receptor A2b. NECA also increased the levels of phosphorylated CRE-binding protein. These findings suggest that adenosine stimulates Nmu expression by activating the cAMP signaling pathway through adenosine receptor A2b in the rat PT. This is the first report to demonstrate that Nmu expression in the PT is regulated by adenosine, which acts as an intravital central metabolic signal, in addition to melatonin, which acts as an external photoperiodic environmental signal. Highlights: A2b is the adenosine receptor expressed in the rat pars tuberalis (PT). Adenosine receptor agonist NECA stimulates Neuromedin U expression in the rat PT. NECA stimulates Neuromedin U expression by activating the cAMP pathway via A2b. NECA promotes Neuromedin U promoter activity via the CRE homology element.