Molecular adaptation in adipose tissue in response to overfeeding with a high-fat diet under sedentary conditions in South Asian and Caucasian men. Issue 3 (14th August 2019)

• Record Type: Journal Article
• Authors: Wulan, Siti N.; Bouwman, Freek G.; Westerterp, Klaas R.; Mariman, Edwin C. M.; Plasqui, Guy
• Is Part Of: British journal of nutrition. Volume 122:Issue 3(2019)
• Page Start: 241
• Page End: 251
• Publication Date: 2019-08-14
• Subjects: Molecular adaptation, -- Overfeeding, -- High-fat diet, -- South Asian men: Caucasian men; Nutrition -- Periodicals; 572.4
• DOI: 10.1017/S0007114519001260
• Languages: English
• ISSNs: 0007-1145

Abstract

Abstract: For the same BMI, South Asians have a higher body fat percentage than Caucasians. There might be differences in the fatty acid (FA) handling in adipose tissue when both ethnicities are exposed to high-fat overfeeding. The objective of the present study was to investigate the molecular adaptation in relation to FA metabolism in response to overfeeding with a high-fat diet (OHFD) in South Asian and Caucasian men. Ten South Asian men (BMI 18–29 kg/m 2 ) and ten Caucasian men (BMI 22–33 kg/m 2 ), matched for body fat percentage, aged 20–40 years were included. A weight-maintenance diet (30 % fat, 55 % carbohydrate and 15 % protein) was given for 3 d followed by 3 d of overfeeding (150 % energy requirement) with a high-fat diet (60 % fat, 25 % carbohydrate and 15 % protein) while staying in a respiration chamber. Before and after overfeeding, abdominal subcutaneous fat biopsies were taken. Proteins were isolated, analysed and quantified for short-chain 3-hydroxyacyl-CoA dehydrogenase (HADH), carnitine palmitoyl-transferase 1α (CPT1a), adipose TAG lipase, perilipin A (PLINA), perilipin B, lipoprotein lipase and fatty acid binding protein 4 using Western blotting. OHFD decreased the HADH level ( P < 0·05) in Caucasians more than in Asians ( P < 0·05), but the baseline and after intervention HADH level was relatively higher in Caucasians. The level of CPT1a decreased in South Asians and increased in Caucasians ( P < 0·05). PLINA did not change with diet but the level was higher in South Asians ( P < 0·05). The observed differences in HADH and PLINA levels as well as in CPT1a response may be important for differences in the long-term regulation of energy (fat) metabolism in these populations.