Development of an UHPLC-MS/MS method for remifentanil quantification in a small plasma volume. Issue 15 (2nd October 2019)

• Record Type: Journal Article
• Authors: O'Hara, Kate; Schneider, Jennifer J.; Jones, Alison L.; Wright, Ian M. R.; Martin, Jennifer H.; Galettis, Peter
• Is Part Of: Journal of liquid chromatography & related technologies. Volume 42:Issue 15/16(2019)
• Page Start: 521
• Page End: 527
• Publication Date: 2019-10-02
• Subjects: HPLC-MS/MS -- LC-MS/MS -- microassay -- neonate -- pharmacokinetics -- remifentanil; Liquid chromatography -- Periodicals; Chromatographic analysis -- Periodicals; Separation (Technology) -- Periodicals; Biotechnology -- Periodicals; Chromatography, Liquid -- Periodicals; 543.8405
• DOI: 10.1080/10826076.2019.1631178
• Languages: English
• ISSNs: 1082-6076

Abstract

Abstract: Remifentanil is a short acting opioid currently used in anesthesia and as an analgesic. This paper describes a simple, fast HPLC-MS/MS methodology that allows detection of remifentanil in low volume plasma samples. Acetonitrile protein precipitation is used for sample extraction and clean up. The assay has a lower limit of detection of 0.25 ng/mL and a 3 min run time. Graphical Abstract: