Autologous transplantation of deciduous tooth pulp into necrotic young permanent teeth for pulp regeneration in a dog model. Issue 10 (October 2019)

• Record Type: Journal Article
• Authors: Huang, Yan; Tang, Xiaoying; Cehreli, Zafer C.; Dai, Xiaoyun; Xu, Jiangjingjun; Zhu, Hongshui
• Is Part Of: Journal of international medical research. Volume 47:Issue 10(2019)
• Page Start: 5094
• Page End: 5105
• Publication Date: 2019-10
• Subjects: Young permanent teeth -- deciduous teeth -- dental pulp -- implant -- pulp revascularization -- autologous transplantation -- regeneration -- dogs -- tooth root -- periapical periodontitis; Medicine -- Periodicals; Pharmacology -- Periodicals; 610.5
• DOI: 10.1177/0300060519862094
• Languages: English
• ISSNs: 0300-0605

Abstract

Objectives: To investigate the potential for pulpal regeneration via autologous transplantation of deciduous tooth pulp into immature necrotic permanent teeth using an experimental dog model. Methods: Experimental apical periodontitis was induced in 60 teeth of six Beagle dogs. Following canal disinfection and pulpotomy, autologous deciduous pulp tissue was transplanted into the root canals (n = 30); as controls, contralateral teeth were treated in accordance with the recommendations of the American Association of Endodontists. Radiographic examinations were performed immediately before transplant, as well as 3 and 6 months after transplant. At the 6-month examination, root samples were collected and histological and immunohistochemical analyses were used to examine tissue regeneration. Results: Radiographic analysis showed no significant differences in most histopathological parameters examined; however, apical diameter reduction was greater in the experimental group. Histological and immunohistochemical analyses showed that the canal walls of the experimental group had newly formed dentin-like tissue with dentinal tubules, while the control group had cementum-like deposits along the canal wall and apical foramina. Conclusions: Autologous transplantation may be useful for regeneration of dental pulp in necrotic young permanent teeth.