Analysis of particle in liquid using excitation-fluorescence spectral flow cytometer. (13th December 2017)

• Record Type: Journal Article
• Authors: Takenaka, Kei; Togashi, Shigenori
• Is Part Of: Japanese journal of applied physics. Volume 57:Number 1(2018)
• Publication Date: 2017-12-13
• Subjects: Physics -- Periodicals; 621.05
• DOI: 10.7567/JJAP.57.017001
• Languages: English
• ISSNs: 0021-4922

Abstract

Abstract: We have developed a new flow cytometer that can measure the excitation-fluorescence spectra of a single particle. This system consists of a solution-transmitting unit and an optical unit. The solution-transmitting unit allows a sample containing particles to flow through the center of a flow cell by hydrodynamic focusing. The optical unit irradiates particles with dispersed white light (wavelength band: 400–650 nm) along the flow direction and measures their fluorescence spectra (wavelength band: 400–700 nm) using a spectroscopic photodetector array. The fluorescence spectrum of a particle changes with the shift of the wavelength of the excitation light. Using this system, the excitation-fluorescence spectra of a fluorescent particle were measured. Additionally, a homogenized tomato suspension and a homogenized spinach suspension were measured using the system. Measurement results show that it is possible to determine the components of vegetables by comparing measured fluorescence spectra of particles in a vegetable suspension.