The hepatic lectin of zebrafish binds a wide range of bacteria and participates in immune defense. Issue 82 (November 2018)

• Record Type: Journal Article
• Authors: Yang, Qingyun; Wang, Peng; Wang, Su; Wang, Yashuo; Feng, Shuoqi; Zhang, Shicui; Li, Hongyan
• Is Part Of: Fish & shellfish immunology. Issue 82(2018)
• Page Start: 267
• Page End: 278
• Publication Date: 2018-11
• Subjects: Zebrafish -- Danio rerio -- C-type lectin -- Hepatic lectin -- Innate immunity; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2018.08.012
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: C-type lectins (CTLs) have a diverse range of functions including cell-cell adhesion, immune response to pathogens and apoptosis. Asialoglycoprotein receptor (ASGPR), also known as hepatic lectin, a member of CTLs, was the first animal lectin identified, yet information regarding it remains rather limited in teleost. In this study, we identified a putative protein in zebrafish, named as the zebrafish hepatic lectin (Zhl). The zhl encoded a typical Ca 2+ -dependent carbohydrate-binding protein, and was mainly expressed in the liver in a tissue specific fashion. Challenge with LPS and LTA resulted in significant up-regulation of zhl expression, suggesting involvement in immune response. Actually, recombinant C-type lectin domain (rCTLD) of Zhl was found to be capable of agglutinating and binding to both Gram-negative and Gram-positive bacteria and enhancing the phagocytosis of the bacteria by macrophages. Moreover, rCTLD specifically bound to insoluble lipopolysaccharide (LPS), lipoteichoic acid (LTA) and peptidoglycan (PGN), which were inhibited by galactose. Interestingly, Zhl was located in the membrane, and its overexpression could inhibit the production of pre-inflammatory cytokines. Taken together, these results indicate that Zhl has immune activity capable of defending invading pathogens, enriching our understanding of the function of ASGPR/hepatic lectin. Highlights: The zebrafish hepatic lectin (Zhl), a homolog of ASGPR(also known as hepatic lectin) is first identified in teleost. The zebrafish is immunocompetent to LPS/LTA challenge via up-regulating the expression of Zhl. Zhl is capable of agglutinating and binding to bacteria and enhancing the phagocytosis of the bacteria by macrophages. Overexpression of zhl could inhibit the production of pre-inflammatory cytokines.