An avirulent Micropterus salmoides rhabdovirus vaccine candidate protects Chinese perch against rhabdovirus infection. Issue 77 (June 2018)

• Record Type: Journal Article
• Authors: Lijuan, Zhang; Ningqiu, Li; Qiang, Lin; Lihui, Liu; Hongru, Liang; Zhibin, Huang; Xiaozhe, Fu
• Is Part Of: Fish & shellfish immunology. Issue 77(2018)
• Page Start: 474
• Page End: 480
• Publication Date: 2018-06
• Subjects: Rhabdovirus -- MSRV-SS -- Chinese perch -- Live vaccine -- Relative percent survival; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2018.03.047
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: In order to develop live vaccine against Siniperca chuatsi rhabdovirus (SCRV) disease, an avirulent virus strain, designed as Micropterus salmoides rhabdovirus Sanshui (MSRV-SS), was selected from six fish rhabdovirus isolates (SCRV-QY、SCRV-SS、SCRV-GM、CMRV-FS、OMBRV-JM、MSRV-SS) by fish challenge assay. When Chinese perch ( Siniperca chuatsi ) were intraperitoneally injected live virus strain MSRV-SS, they were completely protected from virulent SCRV-GM challenge with a relative percent survival (RPS) of 100% on 18th day post vaccination. Then, the wild type MSRV-SS was purified by plaque clone assays, and the biological characteristics of the clonal strain designed as MSRV-SS-7 were investigated. The MSRV-SS-7 was avirulent to Chinese perch and its growth characteristic was similar to the MSRV-SS. The immune protection effects of clonal MSRV-SS-7 against virulent SCRV-GM were evaluated by intraperitoneal injection (IP) vaccination and immersion (IM) vaccination, their RPSs were all 100%. Altogether, these results indicate that MSRV-SS-7 is a potential live vaccine candidate against SCRV disease. Highlights: A natural rhabdoviruses avirulent strain MSRV-SS was picked out and its antigenicity was determined. The clonal strain MSRV-SS-7 was obtained by plaque purification and its bio-characteristics were determined. Live MSRV-SS-7 protected Chinese perch against virulent SCRV by intraperitoneal injection or immersion immunization.