Sylimarin as hepatic protector and immunomodulator in Nile tilapia during Streptococcus agalactiae infection. Issue 82 (November 2018)

• Record Type: Journal Article
• Authors: Owatari, Marco Shizuo; Alves Jesus, Gabriel Fernandes; Brum, Aline; Pereira, Scheila Anelise; Lehmann, Nicollas Breda; de Pádua Pereira, Ulisses; Martins, Maurício Laterça; Pedreira Mouriño, José Luiz
• Is Part Of: Fish & shellfish immunology. Issue 82(2018)
• Page Start: 565
• Page End: 572
• Publication Date: 2018-11
• Subjects: Fish -- Liver protection -- Milk thistle -- Immune stimulation -- Oxidative stress -- Streptococcosis; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2018.08.061
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: This study investigated the use of silymarin, an extract obtained from the milk thistle ( Silybum marianum) and its effects as a possible hepatoprotector in Nile tilapia ( Oreochromis niloticus) . Silymarin was used as feed additive to the diet at a concentration of 0.1% (1 kg per ton of dry ration) with the commercial product named Di-Heptarine S ® (16% silymarin phosphatide). A total of 90 juvenile tilapia with approximately 45 days old and mean weight of 0.72 ± 0.04 g were distributed in two groups, one fed with a diet with the hepatoprotector and the other without the additive. At the end of the assay (55 days after feeding), samples of blood were collected for hematological, immunological, histological (liver, spleen and intestine) and enzymatic analysis such as superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST). After 55 days all fish were challenged with Streptococcus agalactiae serotype Ib to verify the sylimarin effects on the immunological parameters and its protection effect while challenged. During the challenge period another biological material sample was collected for hematological, immunological and histopathological analysis (liver, spleen and intestine). Before the challenge, an increase on the count of thrombocyte was found in the supplemented fish. In the liver, dilation of the sinusoids was observed in unsupplemented fish while supplemented fish the alteration was less severe. No significant alteration was found in SOD, CAT and GST between the groups. Histological changes after the challenge were provoked by bacterial toxins as a result of inflammatory processes. Periacinar degeneration was less intense in unsupplemented fish when compared to supplemented fish. On the other hand, eosinophilic and lymphocytic infiltrate did occur in unsupplemented fish differently from supplemented fish which did not show the alteration. The survival was 28% higher in silymarin supplemented fish when compared to unsupplemented fish that presented no survival. Silymarin supplementation in the diet provided a hepatoprotective and immunomodulatory effect on Nile tilapia. Highlights: First study of the effects of silymarin on hepatic protection and health aspects of Nile tilapia. This is an unprecedented complete study involving a dietary hepatic protector to Nile tilapia. Histological analyzes showed protection on alterations in the organs caused by the ingestion of silymarin. The Silymarin as a dietary feed additive developed a plus immunomodulatory framework with hepatoprotective effects in fish.