Antimicrobial activity of Bulbothrix setschwanensis (Zahlbr.) Hale lichen by cell wall disruption of Staphylococcus aureus and Cryptococcus neoformans. (February 2018)

• Record Type: Journal Article
• Authors: Maurya, Indresh K.; Singh, Samer; Tewari, Rupinder; Tripathi, Manish; Upadhyay, Shashi; Joshi, Yogesh
• Is Part Of: Microbial pathogenesis. Volume 115(2018)
• Page Start: 12
• Page End: 18
• Publication Date: 2018-02
• Subjects: Lichen -- Antibacterial -- Antifungal -- Checkerboard assay -- Mechanistic study -- Mammalian cytotoxicity; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2017.12.015
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: In the present study, antimicrobial activity of a common Himalayan lichen viz. Bulbothrix setschwanensis (Zahlbr.) Hale extract in three common solvents (acetone, chloroform and methanol) was evaluated against six bacterial and seven fungal clinical strains. The acetone extract showed promising antimicrobial activity against S. aureus (1.56 mg/mL) and C. neoformans (6.25 mg/mL). Further, GC-MS analysis revealed 2, 3-bis(2-methylpentanoyloxy)propyl 2-methylpentanoate and Ethyl 2-[(2R, 3R, 4aR, 8aS)-3-hydroxy-2, 3, 4, 4a, 6, 7, 8, 8a-octahydropyrano [3, 2-b]pyran-2-yl]acetate as the predominant compounds. The combination of acetone extract with antibacterial drugs [kanamycin (KAN), rifampicin (RIF)] and antifungal drugs [amphotericin B (Amp B) and fluconazole (FLC)] showed lysis of S. aureus and C. neoformans at non-inhibitory concentration (FICI values were 0.31 for KAN, 0.18 for RIF, 0.37 for Amp B and 0.30 for FLC, respectively). Notably, the acetone extract confirmed cell wall damage of both S. aureus and C. neoformans cells and was clearly visualized under scanning electron microscopy (SEM), flow cytometry and confocal microscopy. Besides this, the three extracts also have less significant cytotoxic activity at MIC concentrations against mammalian cells (HEK-293 and HeLa). This study for the first time suggests that the chemical compounds present in the acetone extract of B. setschwanensis could be used against S. aureus and C. neoformans infections. Graphical abstract: Image 1 Highlights: Bulbothrix setschwanensis ( B. setschwanensis ) lichen extracts showed broad spectrum antimicrobial activity. The extract showed potent synergism with antibiotics against Staphylococcus aureus and Cryptococcus neoformans. Mechanism of action of B. setschwanensis extract has been elucidated.