Tadalafil, a phosphodiesterase type 5 inhibitor, improves bladder blood supply and restores the initial phase of lower urinary tract dysfunction in diabetic rats. Issue 2 (7th August 2017)

• Record Type: Journal Article
• Authors: Gotoh, Daisuke; Torimoto, Kazumasa; Tatsumi, Yoshihiro; Hori, Shunta; Yamada, Atsushi; Miyake, Makito; Morizawa, Yosuke; Aoki, Katsuya; Tanaka, Nobumichi; Hirayama, Akihide; Fujimoto, Kiyohide
• Is Part Of: Neurourology and urodynamics. Volume 37:Issue 2(2018:Feb.)
• Page Start: 666
• Page End: 672
• Publication Date: 2017-08-07
• Subjects: bladder -- ischemia -- phosphodiesterase 5 -- rat; Urinary organs -- Periodicals; Urodynamics -- Periodicals; Urology -- Periodicals; 616.6
• DOI: 10.1002/nau.23372
• Languages: English
• ISSNs: 0733-2467

Abstract

Abstract : Aims: To investigate the effect of tadalafil on bladder blood flow and lower urinary tract function in a rat model of diabetes. Materials and Methods: We studied female Sprague‐Dawley rats and induced diabetes in some using a single intraperitoneal injection of streptozotocin. We divided the rats into nondiabetes (ND), diabetes (D), and diabetes with tadalafil (DT) groups. The rats were raised for an additional 7 weeks after diabetes induction. The DT group received oral tadalafil (2 mg/kg/day) for 7 days before the experiments. At 7 weeks after diabetes induction, we performed cystometry, resected the bladders for immunohistochemistry (hypoxia‐inducible factor‐1α [HIF‐1α] and 8‐oxo‐2′‐deoxyguanosine [8‐OHdG] staining), and measured bladder blood supply using a laser blood flow meter. Results: The opening pressure, when the urethra opens and urine flow starts, was significantly lower in the DT group than in the D group (24.9 ± 5.9 vs 43.6 ± 12.3 cmH2 O). The inter‐contraction interval was significantly longer in the D group than in the ND and DT groups (1566.2 ± 168.7 vs 702.9 ± 165.2 and 787.4 ± 148.8 s). Immunohistochemistry showed positive staining of the urothelial layer for both HIF‐1α and 8‐OHdG in the D group, but not in the ND or DT groups. Bladder blood flow was significantly lower in the D group than in the ND or DT groups. Conclusions: Tadalafil improves bladder blood supply and lower urinary tract function in diabetic rats. Tadalafil may be a promising drug that restores lower urinary tract dysfunction in the early phase of diabetes.