Assessment of deoxynivalenol exposure among Bangladeshi and German adults by a biomarker-based approach. (6th September 2016)

• Record Type: Journal Article
• Authors: Ali, Nurshad; Blaszkewicz, Meinolf; Degen, Gisela H.
• Is Part Of: Toxicology letters. Volume 258(2016)
• Page Start: 20
• Page End: 28
• Publication Date: 2016-09-06
• Subjects: Biomonitoring -- Deoxynivalenol -- Deepoxy-deoxynivalenol -- Exposure -- Mycotoxins -- Urine; Toxicology -- Periodicals; 363.179
• DOI: 10.1016/j.toxlet.2016.06.006
• Languages: English
• ISSNs: 0378-4274

Abstract

Highlights: Deoxynivalenol (DON) biomarker analysis in urines of Bangladeshi adults document low exposure to this mycotoxin. Biomarker levels in German samples reveal more frequent and considerably higher dietary DON intake of German adults. Yet, presently DON intake in German adults is below the provisional maximal tolerable daily intake (PMTDI) value set for this mycotoxin. The new results are discussed in relation to data from other countries which indicate that the PMTDI for DON can be exceeded in parts of the population. Abstract: Deoxynivalenol (DON) is a frequent mycotoxin contaminant in cereal crops worldwide and can cause adverse health effects in exposed animals and humans. Since DON contamination in Bangladeshi food is unexplored, we conducted a biomonitoring study to assess DON exposure in the Bangladeshi population and compare it with that of German adults. In total 214 urines were collected, n = 164 in Bangladesh and n = 50 in Germany. In Bangladesh rural and urban residents of Rajshahi district provided urines in two seasons (n = 69 in summer, n = 95 in winter, with 62 participants enrolled in both periods). Urinary DON and its de-epoxy metabolite DOM-1 were measured by a previously validated sensitive LC–MS/MS method. In Bangladeshi urines, DON was detectable in 27% (range 0.16–1.78 ng/mL) in summer and 31% (range 0.16–1.21 ng/mL) in winter season. There was no significant difference at the mean DON level between season (summer 0.17 ± 0.25 ng/mL and winter 0.16 ± 0.18 ng/mL) and region (rural or urban residents). The metabolite DOM-1 was not detected in any urine from Bangladesh. In contrast, DON and DOM-1 were detected in 100% (range 0.16–38.44 ng/mL) and 40% (range 0.10–0.73 ng/mL), respectively, of the German urines. The mean DON level in German urines (9.02 ± 6.84 ng/mL) was about 53-fold higher than that found in Bangladeshi samples. This indicates a low and high dietary DON exposure among the adult population in Bangladesh and Germany, respectively. The biomarker concentrations found and published urinary excretion rates for DON then served to calculate the daily mycotoxin intake in both cohorts: the mean DON intake in Bangladesh being 6 ng/kg b.w., and in Germany a mean of 268 and maximum intake of 975 ng/kg b.w., values lower than the provisional maximum tolerable daily intake of 1 μg/kg b.w. set by the WHO/JECFA.