Design of Hollow Protein Nanoparticles with Modifiable Interior and Exterior Surfaces. Issue 38 (24th August 2018)

• Record Type: Journal Article
• Authors: Kawakami, Norifumi; Kondo, Hiroki; Matsuzawa, Yuki; Hayasaka, Kaoru; Nasu, Erika; Sasahara, Kenji; Arai, Ryoichi; Miyamoto, Kenji
• Is Part Of: Angewandte Chemie international edition. Volume 57:Issue 38(2018)
• Page Start: 12400
• Page End: 12404
• Publication Date: 2018-08-24
• Subjects: cage proteins -- fusion protein -- nanoparticles -- protein design -- self-assembly; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201805565
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Protein‐based nanoparticles hold promise for a broad range of applications. Here, we report the production of a uniform anionic hollow protein nanoparticle, designated TIP60, which spontaneously assembles from a designed fusion protein subunit based on the geometric features of polyhedra. We show that TIP60 tolerates mutation and both its interior and exterior surfaces can be chemically modified. Moreover, TIP60 forms larger structures upon the addition of a cationic protein. Therefore, TIP60 can be used as a modifiable nano‐building block for further molecular assembly. Abstract : An anionic protein nanoparticle having truncated icosahedral shape is assembled from an artificial fusion protein that is designed according to Euler's polyhedron formula. The protein nanoparticle can be modified by a small molecule at interior and exterior surfaces. The addition of a cationic protein initiates formation of a larger structure. These features allow its use as a chemically modifiable nano‐building block.