Generation of the UFM1 Toolkit for Profiling UFM1‐Specific Proteases and Ligases. Issue 43 (1st October 2018)

• Record Type: Journal Article
• Authors: Witting, Katharina F.; van der Heden van Noort, Gerbrand J.; Kofoed, Christian; Talavera Ormeño, Cami; el Atmioui, Dris; Mulder, Monique P. C.; Ovaa, Huib
• Is Part Of: Angewandte Chemie international edition. Volume 57:Issue 43(2018)
• Page Start: 14164
• Page End: 14168
• Publication Date: 2018-10-01
• Subjects: activity-based probes -- chemical biology -- native chemical ligation -- post-translational modifications -- UFM1; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201809232
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Ubiquitin‐fold modifier 1 (UFM1) is a reversible post‐translational modifier that is covalently attached to target proteins through an enzymatic cascade and removed by designated proteases. Abnormalities in this process, referred to as Ufmylation, have been associated with a variety of human diseases. Given this, the UFM1‐specific enzymes represent potential therapeutic targets; however, understanding of their biological function has been hampered by the lack of chemical tools for activity profiling. To address this unmet need, a diversifiable platform for UFM1 activity‐based probes (ABPs) utilizing a native chemical ligation (NCL) strategy was developed, enabling the generation of a variety of tools to profile both UFM1 conjugating and deconjugating enzymes. The use of the probes is demonstrated in vitro and in vivo for monitoring UFM1 enzyme reactivity, opening new research avenues. Abstract : Introducing the UFM1 toolkit : A facile native chemical ligation strategy to enable the development of UFM1 reagents and activity‐based probes is presented. This toolkit permits the study of both UFM1‐specific proteases and conjugating enzymes in vitro and in cells, thereby opening new research avenues for the discovery of specific UFM1 inhibitors.