A Method for Routine Analysis of Urinary 4, 4′‐methylenebis (2‐chloroaniline) by Gas Chromatography‐Electron Capture Detection. Issue 5 (22nd November 2014)

• Record Type: Journal Article
• Authors: Takeuchi, Akito; Namera, Akira; Sakui, Norihiro; Yamamuro, Kenji; Kawai, Toshio; Sumino, Kimiaki
• Is Part Of: Journal of occupational health. Volume 56:Issue 5(2014)
• Page Start: 347
• Page End: 350
• Publication Date: 2014-11-22
• Subjects: 4, 4'‐Methylenebis (2‐chloroaniline) -- Biological monitoring -- Gas chromatography‐electron capture detection -- Urine; Medicine, Industrial -- Periodicals; Industrial hygiene -- Periodicals; Medicine, Industrial; Occupational Diseases; Occupational Exposure; Occupational Health; Occupational Medicine; Periodicals; Periodical; Electronic journals; 613.62
• DOI: 10.1539/joh.14-0052-BR
• Languages: English
• ISSNs: 1341-9145

Abstract

Abstract : A Method for Routine Analysis of Urinary 4, 4'‐methylenebis (2‐chloroaniline) by Gas Chroma‐tography‐Electron Capture Detection: Akito TAKEU‐CHI, et al . Osaka Occupational Health Service Center, Japan Industrial Safety and Health Association— Objectives: The purpose of this study was to develop a simple and cost‐effective method for the determination of urinary 4, 4'‐methylenebis (2‐chloroaniline) (MBOCA) by gas chromatography‐electron capture detection (GC‐ECD) for biological monitoring of occupational exposure to MBOCA. Methods: MBOCA was prepared by liquidliquid extraction after alkaline hydrolysis, derivatized with N ‐methyl‐bis (trifluoroacetamide) and then analyzed using GC‐ECD. The proposed method was validated in accordance with the US Food and Drug Administration guidance. Results: The calibration curve showed linearity in the range 1–100 μg/ l, with a correlation coefficient of >0.999. The limits of detection and quantification were 0.3 μg/ l and 1 μg/ l, respectively. The recovery was 94−99%. Intraday accuracy, expressed as the deviation from the nominal value, was 90.5−100.3%, and intraday precision, expressed as the relative standard deviation, was 0.3−2.4%. Interday accuracy and precision were 87.8−100.2% and 0.3−4.1%, respectively. Conclusions: The proposed method is a simple and cost‐effective method suitable for routine analyses and could be useful for biological monitoring of occupational exposure to MBOCA.