Enhancement of sucrose metabolism in Clostridium saccharoperbutylacetonicum N1-4 through metabolic engineering for improved acetone–butanol–ethanol (ABE) fermentation. (December 2018)

• Record Type: Journal Article
• Authors: Zhang, Jie; Wang, Pixiang; Wang, Xiaofei; Feng, Jun; Sandhu, Hardev S.; Wang, Yi
• Is Part Of: Bioresource technology. Volume 270(2018)
• Page Start: 430
• Page End: 438
• Publication Date: 2018-12
• Subjects: Clostridium saccharoperbutylacetonicum -- Sucrose metabolism -- Metabolic engineering -- Acetone–butanol–ethanol fermentation -- Sugarcane juice; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2018.09.059
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: PTS system is the primary sucrose catabolic pathway in C. saccharoperbutylacetonicum . Colloid-like polysaccharide was generated after inactivation of sucrose operon. Deletion of scrR alleviated CCR effect and enhanced sucrose consumption. Overexpressing native sucrose pathway enhanced sucrose utilization & ABE production. Sugarcane juice can be used as an inexpensive substrate for ABE fermentation. Abstract: This work investigated sucrose metabolism in C. saccharoperbutylacetonicum . Inactivation of sucrose catabolism operon resulted in 28.9% decrease in sucrose consumption and 44.1% decrease in ABE production with sucrose as sole carbon source. Interestingly, a large amount of colloid-like polysaccharides were generated in the mutant, which might be due to inefficient intracellular sucrose metabolism. Deletion of transcriptional repressor gene successfully alleviated CCR and enhanced ABE production by 24.7%. Additional overexpression of endogenous sucrose pathway further elevated sucrose consumption and enhanced ABE production by 17.2%, 45.7%, or 22.5% compared to wild type with sucrose, mixed sugars or sugarcane juice as substrate, respectively. The engineered strain could be a robust platform for efficient biofuel production from inexpensive sucrose-based carbon sources.