CATH-2 and LL-37 increase mannose receptor expression, antigen presentation and the endocytic capacity of chicken mononuclear phagocytes. (October 2017)

• Record Type: Journal Article
• Authors: Kraaij, Marina D.; van Dijk, Albert; Haagsman, Henk P.
• Is Part Of: Molecular immunology. Volume 90(2017:Oct.)
• Page Start: 118
• Page End: 125
• Publication Date: 2017-10
• Subjects: Host defense peptide -- Cathelicidin -- Antigen presentation -- Immunemodulation -- Chicken; Immunochemistry -- Periodicals; Molecular biology -- Periodicals; Immunochemistry -- Periodicals; Allergy and Immunology -- Periodicals; Molecular Biology -- Periodicals; Immunochimie -- Périodiques; Biologie moléculaire -- Périodiques; Immunochemistry; Molecular biology; Periodicals; Electronic journals; 571.96
• DOI: 10.1016/j.molimm.2017.07.005
• Languages: English
• ISSNs: 0161-5890

Abstract

Highlights: L-CATH-2, D-CATH-2 and LL-37 increase mannose receptor expression. L-CATH-2, D-CATH-2 and LL-37 increase antigen presentation. L-CATH-2, D-CATH-2 and LL-37 increase endocytosis. L-CATH-2, D-CATH-2 and LL-37 modulate the immune response of chicken immune cells. Abstract: Cathelicidins display in vitro and in vivo immunomodulatory activities and are part of the innate immune system. Previously, we found that in ovo treatment with chicken cathelicidin CATH-2 partially protects young broilers against respiratory E. coli infection. To determine the cellular aspects of this protection, we investigated immunomodulatory effects of CATH-2 and the human cathelicidin LL-37 on primary chicken peripheral blood mononuclear cells (PBMCs). Treatment of chicken PBMCs with L-CATH-2, D-CATH-2 or LL-37 increased the percentage of mononuclear phagocytes, but decreased that of B cells. L-CATH-2, D-CATH-2 and LL-37 treatment of chicken PBMCs also enhanced the expression levels of mannose receptor MRC1 and antigen presentation markers MHCII, CD40 and CD86 on mononuclear phagocytes, indicating increased antigen presentation capacity. Concomitantly, L-CATH-2, D-CATH-2 and LL-37 neutralized LPS-induced cytokine production, while increasing the endocytic capacity. We conclude that L-CATH-2, D-CATH-2 and LL-37 can modulate the immune response of primary chicken immune cells by increasing mannose receptor expression, antigen presentation, endocytosis and neutralizing LPS-induced cytokine production and as a result augment activation of the adaptive immune system.