PcToll3 was involved in anti-Vibrio response by regulating the expression of antimicrobial peptides in red swamp crayfish, Procambarus clarkii. (October 2016)

• Record Type: Journal Article
• Authors: Lan, Jiang-Feng; Wei, Shun; Wang, Yu-Qing; Dai, Yun-Jia; Tu, Jia-Gang; Zhao, Li-Juan; Li, Xin-Cang; Qin, Qi-Wei; Chen, Nan; Lin, Li
• Is Part Of: Fish & shellfish immunology. Volume 57(2016:Oct.)
• Page Start: 17
• Page End: 24
• Publication Date: 2016-10
• Subjects: Red swamp crayfish -- PcToll3 -- Antimicrobial peptides -- PcDorsal; MyD88 myeloid differentiation factor 88 -- TRAF6 tumor necrosis factor-associated factor 6 -- NF-κB nuclear factor kappa B -- IRF interferon-regulatory factors -- ALF1 Anti-lippopolysaccharide factor 1 -- Cru1 Crustin1 -- Lys1 Lysozymes1 -- NBT nitrotetrazolium blue chloride -- BCIP 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2016.08.021
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Tolls and Toll-like receptors (TLRs) play an important role in host immune defenses by regulating the expression of antimicrobial peptides (AMPs) and cytokines, but the functional differences of crustacean Tolls from Drosophila Tolls or Mammal TLRs are largely unknown. A novel Toll receptor, named PcToll3, was identified from red swamp crayfish, Procambarus clarkii . It was widely expressed in all detected tissues, and its transcript in hemocytes was up-regulated at 12 h after Vibrio parahemolyticus ( Vibrio ) injection or at 24 h post white spot syndrome virus (WSSV) challenge. After knockdown of PcToll3, the activity of bacterial clearance was inhibited, and the expression levels of AMPs including Crustin1 ( Cru1 ), Anti-lippopolysaccharide factor 1 ( ALF1 ), and Lysozymes1 ( Lys1 ), which could be up-regulated by Vibrio, were all affected. Meanwhile, PcToll3 silencing influenced the expression of myeloid differentiation factor 88 ( PcMyd88 ), tumor necrosis factor-associated factor 6 ( PcTRAF6 ), and PcDorsal, which were the counterparts of Drosophila Toll signaling pathway. Interestingly, PcToll3 silencing inhibited translocation of PcDorsal from cytoplasm to nucleus. Furthermore, the knockdown of PcDorsal also impaired the expression of AMPs after Vibrio challenge. Hence, we concluded that, besides participating in antiviral immunity, PcToll3 might also regulate the expression of Cru1 and Lys1 to participate in anti- Vibrio immune responses by promoting PcDorsal translocation into nucleus. Highlights: The expression of PcToll3 was up-regulated when challenge with Vibrio parahemolyticus. Bacterial clearance rate was decreased in PcToll3 -RNAi crayfish infected by Vibrio. PcToll3 -RNAi decrease the expression levels of Cru1 and Lys1 under challenge with Vibrio. PcToll3 influenced nuclear import of PcDorsal in red swamp crayfish.