Molecular characterization, expression analysis, and bactericidal activity of the derivative peptides of TFPI-1 and TFPI-2 in half-smooth tongue sole, Cynoglossus semilaevis. (November 2016)

• Record Type: Journal Article
• Authors: Zhao, Xin-peng; He, Shu-wen; Yue, Bin; Wang, Guang-hua; Zhang, Min
• Is Part Of: Fish & shellfish immunology. Volume 58(2016:Nov.)
• Page Start: 563
• Page End: 571
• Publication Date: 2016-11
• Subjects: Cynoglossus semilaevis -- TFPI-1 -- TFPI-2 -- Expression -- C-terminal peptides -- Antibacterial activity; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2016.10.003
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Tissue factor pathway inhibitors (TFPIs) are Kunitz-type serine protease inhibitors that reversibly regulate the blood coagulation induced by tissue factor. TFPI family contain two members, TFPI-1 and TFPI-2. Recent studies have shown TFPI-1 and TFPI-2 also play important roles in innate immunity, however, the potential function of teleost TFPI are very limited. In this study, we characterized two TFPI (CsTFPI-1 and CsTFPI-2) molecules from half-smooth tongue sole ( Cynoglossus semilaevis ), examined their tissue distributions and expression patterns under pathogens stimulation as well as investigated the antibacterial activity of the C-terminal peptides. Quantitative real time RT-PCR analysis showed that constitutive CsTFPI-1 expression occurred, in increasing order, in head kidney, intestine, brain, spleen, liver, skin, gills, heart, and muscle; CsTFPI-2 was expressed, in increasing order, in the gills, intestine, skin, head kidney, liver, brain, spleen, muscle, and heart. Under Vibrio anguillarum, Streptococcus agalactiae and fish megalocytivirus stimulation, both CsTFPI-1 and CsTFPI-2 expression increased significantly in a manner that depended on the pathogen, tissue type, and infection stage, which suggested CsTFPI-1 and CsTFPI-2 play important roles in anti-bacterial and anti-viral infection. Finally, C-terminal peptides of CsTFPI-1 and CsTFPI-2, were synthesized and proved to have antibacterial effect against Micrococcus luteus that were independent of host serum. Take together, these results indicate that CsTFPI-1 and CsTFPI-2 play important roles in antimicrobial immunity of this fish. Highlights: Two TFPI homologues (CsTFPI-1 and CsTFPI-2) were characterized. Both CsTFPI-1 and CsTFPI-2 distributed ubiquitously in tongue sole tissues. Both CsTFPI-1 and CsTFPI-2 could be induced by bacterial or viral pathogens. C-terminal peptides of CsTFPI-1 and CsTFPI-2 displayed antibacterial activity that were independent of host serum.