Transplantation of VEGFl65-overexpressing vascular endothelial progenitor cells relieves endothelial injury after deep vein thrombectomy. (January 2016)

• Record Type: Journal Article
• Authors: Tang, Jun-jian; Meng, Qing-you; Cai, Zhi-xin; Li, Xiao-qiang
• Is Part Of: Thrombosis research. Volume 137(2016)
• Page Start: 41
• Page End: 45
• Publication Date: 2016-01
• Subjects: Vascular endothelial growth factor -- Gene transfection -- Endothelial progenitor cells -- Adenovirus -- Vein thrombectomy -- Endothelium injury; Thrombosis -- Periodicals; 616.135
• DOI: 10.1016/j.thromres.2015.11.025
• Languages: English
• ISSNs: 0049-3848

Abstract

Abstract: Aim: The purpose of this study was to explore the therapeutic efficacy of VEGF165-overexpressing vascular endothelial progenitor cells (EPCs) in post-thrombotic syndrome. Materials and methods: A thrombus model was developed to mimic the in-vivo setting, and adenovirus transfection was used to overexpress VEGF165 in EPCs. These cells were transplanted into the animal model, and their ability to relieve endothelial injury was evaluated using haematoxylin and eosin staining, immunohistochemistry and scanning electron microscopy. Results: Ferric chloride was used to build rat models of the inferior vena cava thrombosis, and HEK 293A cells were used to amplify adenovirus that overexpresses VEGF165. EPCs were infected with adenovirus, and this was confirmed by fluorescence microscopy. Transplantation of VEGH165-overexpressing EPCs into injured endothelial sites led to faster repair of the post-thrombotic tunica intima than wild-type EPCs. Conclusion: Transplantation of VEGF165-overexpressing EPCs was found to promote repair of the tunica intima, thus improving rehabilitation after surgery. Highlights: We establish a good rodent model for thrombosis. We separate and cultivate endothelial progenitor cells (EPCs) in vitro. EPCs can relieve endothelium injury after deep vein thrombectomy.