Extracellular polysaccharide derived from potential probiotic strain with antioxidant and antibacterial activities as a prebiotic agent to control pathogenic bacterial biofilm formation. (August 2017)

• Record Type: Journal Article
• Authors: Mahdhi, Abdelkarim; Leban, Nadia; Chakroun, Ibtissem; Chaouch, Mohamed Aymen; Hafsa, Jawhar; Fdhila, Kais; Mahdouani, Kacem; Majdoub, Hatem
• Is Part Of: Microbial pathogenesis. Volume 109(2017)
• Page Start: 214
• Page End: 220
• Publication Date: 2017-08
• Subjects: Exopolysaccharides -- Probiotics -- Antibiofilm -- Antioxidant -- Cytotoxicity; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2017.05.046
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Because of their functional diversity, bioactive compounds are becoming a new biocontrol agent to limit biofilm formation by pathogens. In this study, the physico-chemical characterization of an exopolysaccharide (EPS) isolated from Lactobacillus plantarum (EPLB) was characterized and its in vitro effect on biofilm formation was studied. The EPS had a molecular weight of 36 kDa and polydispersity index estimated to be 1.2. The tested EPLB had an antibacterial activity, with a Minimal Inhibition Concentration (MIC) values ranging between 1 mg/ml and 10 mg/ml, displayed an antibiofilm effect concentration dependent on Gram positive and negative strains. Among the pathogenic strains, 2 out of 4 appeared to be more than 50% inhibited in their biofilm development by the EPS. The antibiofilm activity can be due to the ability of the EPS to influence the function of biological membranes like hydrophobicity that decreased (P < 0.05) when the EPS was used at a concentration of 512 μg/ml. This EPS without cytotoxic effect, showed an antioxidant effect on the quenching of DPPH radicals and the inhibition of lipid peroxidation with a percentage of 64% and 66%, respectively. Taken together these biological properties, EPLB can be considered as a potential prebiotic agent in the design of new therapeutic strategies for bacterial biofilm-associated infections. Abstract : Bacterial extracellular polysaccharide as prebiotic agent to control biofilm. Physico-chemical characterization: sugar determination, (ATR-FTIR), SEC. Noticeable potential as antibacterial and antioxidant agent. Antibiofilm effect (inhibition and eradication) without cytotoxicity.