Imaging of Enzyme Activity by Electron Paramagnetic Resonance: Concept and Experiment Using a Paramagnetic Substrate of Alkaline Phosphatase. Issue 36 (7th August 2018)

• Record Type: Journal Article
• Authors: Sanzhaeva, Urikhan; Xu, Xuan; Guggilapu, Priyaankadevi; Tseytlin, Mark; Khramtsov, Valery V.; Driesschaert, Benoit
• Is Part Of: Angewandte Chemie international edition. Volume 57:Issue 36(2018)
• Page Start: 11701
• Page End: 11705
• Publication Date: 2018-08-07
• Subjects: enzymes -- EPR spectroscopy -- imaging -- nitroxide -- phosphatase; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201806851
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Enzyme activities are well established biomarkers of many pathologies. Imaging enzyme activity directly in vivo may help to gain insight into the pathogenesis of various diseases but remains extremely challenging. In this communication, we report the use of EPR imaging (EPRI) in combination with a specially designed paramagnetic enzymatic substrate to map alkaline phosphatase activity with a high selectivity, thereby demonstrating the potential of EPRI to map enzyme activity. Abstract : Enzyme kinetics by EPR : EPR imaging of enzyme activity has been performed using a specially designed paramagnetic substrate of alkaline phosphatase (ALP). Imaging of ALP activity could lead to new insights into phosphorous metabolism in tumors.