Mapping serum antibody repertoires using peptide libraries. (March 2018)

• Record Type: Journal Article
• Authors: Paull, Michael L; Daugherty, Patrick S
• Is Part Of: Current opinion in chemical engineering. Volume 19(2018)
• Page Start: 21
• Page End: 26
• Publication Date: 2018-03
• Subjects: Chemical engineering -- Periodicals; Chemical engineering; Periodicals; 660.05
• DOI: 10.1016/j.coche.2017.12.001
• Languages: English
• ISSNs: 2211-3398

Abstract

Graphical abstract: Highlights: Serum antibody repertoires can aid efforts to develop vaccines and diagnostics. Peptide libraries can be random or nonrandom using microarrays or surface display. Motif discovery software can identify epitopes from antibody-binding peptides. Individual antibody repertoires are unique, but recognize common epitopes. Abstract : Antibodies in blood provide a rich source of immunological information. Antibody repertoire analysis seeks to decode this information to empower the development of vaccines, diagnostics, and therapeutics. To this end, various approaches have been developed to determine epitopes using peptide libraries. Approaches have used random or proteome-derived peptide libraries in a microarray or surface display format. For methods using random libraries, motif discovery software has been developed to identify common binding signatures. The analysis of thousands of samples and dozens of diseases has shown that there are often disease-specific epitopes, even though individual antibody repertoires are unique. The recent developments in antibody repertoire analysis hold the potential to enable comprehensive immune evaluations.