Bisphenol A and estradiol impede myoblast differentiation through down-regulating Akt signaling pathway. (August 2018)

• Record Type: Journal Article
• Authors: Go, Ga-Yeon; Lee, Sang-Jin; Jo, Ayoung; Lee, Jae-Rin; Kang, Jong-Sun; Yang, Mihi; Bae, Gyu-Un
• Is Part Of: Toxicology letters. Volume 292(2018)
• Page Start: 12
• Page End: 19
• Publication Date: 2018-08
• Subjects: Bisphenol A -- Estradiol -- Myoblast differentiation -- Akt signaling; Toxicology -- Periodicals; 363.179
• DOI: 10.1016/j.toxlet.2018.04.019
• Languages: English
• ISSNs: 0378-4274

Abstract

Graphical abstract: Highlights: Bisphenol A (BPA) suppresses muscle-specific gene expression and myotube formation. BPA inhibits Akt signaling pathway, reducing myoblast differentiation. Estradiol attenuates myogenesis through inhibiting Akt signaling. Abstract: Bisphenol A (BPA), one of the most widespread endocrine disrupting chemicals, is known as an artificial estrogen, which interacts with estrogen receptor (ER). In this study, we investigated the effects of BPA and estradiol on myoblast differentiation and the underlying signaling mechanism. Exposure to BPA (0.01–1 μM) in mouse myoblast C2C12 cells attenuated myogenic differentiation via the reduced expression of muscle-specific genes, such as myosin heavy chain ( MHC ), MyoD, and Myogenin, without the alteration of cell proliferation and viability. BPA-exposed C2C12 myoblasts also showed a reduction of Akt phosphorylation ((37–61) %, p < 0.001), a key event for myogenesis. Similarly to BPA, estradiol (0.01–1 μM) reduced the expression of muscle-specific proteins and the formation of multinucleated myotubes, and attenuated the muscle differentiation-specific phosphorylation of Akt ((42–59) %, p < 0.001). We conclude that BPA and estradiol suppress myogenic differentiation through the inhibition of Akt signaling.