Neutrophil Secretion Induced by an Intracellular Ca2+ Rise and Followed by Whole-Cell Patch-Clamp Recordings Occurs Without any Selective Mobilization of Different Granule Populations. (4th June 2006)

• Record Type: Journal Article
• Authors: Granfeldt, Daniel; Harbecke, Olle; Björstad, Åse; Karlsson, Anna; Dahlgren, Claes
• Is Part Of: Journal of biomedicine and biotechnology. Volume 2006(2006)
• Publication Date: 2006-06-04
• Subjects: Medicine -- Periodicals; Biology -- Periodicals; Biotechnology -- Periodicals; Medicine; Biology; Biotechnology; Médecine; Biologie; Biotechnologie; Biology; Biotechnology; Medicine; Biotechnology; Biomedicine; Electronic journals; Periodical; Periodicals; Electronic journals; 610
• DOI: 10.1155/JBB/2006/97803
• Languages: English
• ISSNs: 1110-7243

Abstract

Abstract : We have investigated calcium-induced secretion in human neutrophils, using a whole-cell patch-clamp technique. Mobilization of subcellular granules to the cell membrane was followed as the change in membrane capacitance (△ C m ). Both the magnitude and the kinetics of the response differed between low and high concentrations of Ca2 + . A sustained secretion following a short lag phase was induced by high concentrations of Ca2 + (100μ M and higher). A stable plateau was reached after 5–7 minutes at△ C m values corresponding to values expected after all specific as well as azurophil granules have been mobilized. Capacitance values of the same magnitude could be obtained also at lower Ca2 + concentrations, but typically no stable plateau was reached within the measuring time. In contrast to previous studies, we were unable to detect any pattern of secretion corresponding to a distinct submaximal response or selective mobilization of granule subsets specified by their Ca2 + -sensitivity.