Pluripotent stem cells as a source of osteoblasts for bone tissue regeneration. (March 2019)

• Record Type: Journal Article
• Authors: Zhu, Hui; Kimura, Takaharu; Swami, Srilatha; Wu, Joy Y.
• Is Part Of: Biomaterials. Volume 196(2019)
• Page Start: 31
• Page End: 45
• Publication Date: 2019-03
• Subjects: Embryonic stem cells -- Induced pluripotent stem cells -- Osteoblasts -- Differentiation -- Tissue engineering; Biomedical materials -- Periodicals; Biocompatible Materials -- Periodicals; Biomatériaux -- Périodiques; 610.28
• DOI: 10.1016/j.biomaterials.2018.02.009
• Languages: English
• ISSNs: 0142-9612

Abstract

Abstract: Appropriate and abundant sources of bone-forming osteoblasts are essential for bone tissue engineering. Pluripotent stem cells can self-renew and thereby offer a potentially unlimited supply of osteoblasts, a significant advantage over other cell sources. We generated mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) from transgenic mice expressing rat 2.3 kb type I collagen promoter-driven green fluorescent protein (Col2.3GFP), a reporter of the osteoblast lineage. We demonstrated that Col2.3GFP ESCs and iPSCs can be successfully differentiated to osteoblast lineage cells that express Col2.3GFP in vitro . We harvested GFP + osteoblasts differentiated from ESCs. Genome wide gene expression profiles validated that ESC- and iPSC-derived osteoblasts resemble calvarial osteoblasts, and that Col2.3GFP expression serves as a marker for mature osteoblasts. Our results confirm the cell identity of ESC- and iPSC-derived osteoblasts and highlight the potential of pluripotent stem cells as a source of osteoblasts for regenerative medicine.