Identifying genes progressively silenced in preneoplastic and neoplastic liver tissues. (6th August 2010)

• Record Type: Journal Article
• Authors: Archer, Kellie J.; Zhao, Zhongming; Guennel, Tobias; Maluf, Daniel G.; Fisher, Robert; Mas, Valeria
• Is Part Of: International journal of computational biology and drug design. Volume 3:Number 1(2010)
• Page Start: 52
• Page End: 67
• Publication Date: 2010-08-06
• Subjects: HCC -- hepatocellular carcinoma -- HCV -- hepatitis C virus -- methylation -- microarray -- hepatocarcinogenesis -- liver tissues -- bioinformatics -- gene silencing -- malignant hepatoma -- liver cancer; Computational biology -- Periodicals; Drugs -- Design -- Periodicals; 570.285
• Languages: English
• ISSNs: 1756-0756

Abstract

High-throughput genomic technologies are increasingly being used to identify therapeutic targets and risk factors for specific diseases. Using 116 independent liver samples, we identified 793 probe sets that demonstrated a significant association in the frequency of absent calls as tissues progressed from normal to pre-neoplastic to neoplastic, followed by a bioinformatic approach which identified that 78.9% of the significant probe sets contained at least one CpG island in the gene promoter region compared with 58.9% of the remaining genes examined. Our results indicate that further high-throughput methylation studies to more fully characterize molecular events involved in hepatocarcinogenesis are warranted.