6, 7-dimethoxy-coumarin as a probe of hydration dynamics in biologically relevant systems. (12th February 2018)

• Record Type: Journal Article
• Authors: Ghose, Avisek; Amaro, Mariana; Kovaricek, Petr; Hof, Martin; Sykora, Jan
• Is Part Of: Methods and applications in fluorescence. Volume 6:Number 2(2018:Jun.)
• Publication Date: 2018-02-12
• Subjects: time dependent fluorescence shift -- 6 -- 7-dimethoxy-coumarin -- hydration -- water dynamics -- reverse micelles; Fluorescence spectroscopy -- Periodicals; Fluorescence -- Periodicals; Spectrometry, Fluorescence -- Periodicals; Fluorescence -- Periodicals; Fluorescence; Fluorescence spectroscopy; Periodicals; 535.352
• DOI: 10.1088/2050-6120/aaaa05
• Languages: English
• ISSNs: 2050-6120

Abstract

Abstract: Coumarin derivatives are well known fluorescence reporters for investigating biological systems due to their strong micro-environment sensitivity. Despite having wide range of environment sensitive fluorescence probes, the potential of 6, 7-dimethoxy-coumarin has not been studied extensively so far. With a perspective of its use in protein studies, namely using the unnatural amino acid technology or as a substrate for hydrolase enzymes, we study acetyloxymethyl-6, 7-dimethoxycoumarin (Ac-DMC). We investigate the photophysics and hydration dynamics of this dye in aerosol-OT (AOT) reverse micelles at various water contents using the time dependent fluorescence shift (TDFS) method. The TDFS response in AOT reverse micelles from water/surfactant ratio of 0 to 20 confirms its sensitivity towards the hydration and mobility of its microenvironment. Moreover, we show that the fluorophore can be efficiently quenched by halide ions. Hence, we conclude that the 6, 7-dimethoxy-methylcoumarin fluorophore is useful for studying hydration parameters in biologically relevant systems.