An efficient chemodosimeter for Cu(II) ions based on hydrolysis of fluorescein and its utility in live cell imaging. (December 2019)

• Record Type: Journal Article
• Authors: Wang, Xin; Shao, Juanjuan; Hu, Yangfeng
• Is Part Of: Dyes and pigments. Volume 171(2019)
• Publication Date: 2019-12
• Subjects: Copper(II) sensor -- Fluorescein -- Turn-on -- Imaging -- Chemodosimeter; Dyes and dyeing -- Periodicals; Pigments -- Periodicals; 667.2
• DOI: 10.1016/j.dyepig.2019.107680
• Languages: English
• ISSNs: 0143-7208

Abstract

Abstract: A new NBD-based chemodosimeter system bearing fluorescein derivative has been synthesized for the detection of Cu(II) ions. The spirocyclic form of molecular system bearing a cleavable active bond performs as a fluorescent probe for Cu 2+ ion selectively towards other metal ions. The sensing mechanism of Cu 2+ with fluorescein hydrazide supports hydrolytic cleavage of the amide bond, hence causing the release of fluorophore (fluorescein) and enhancement of fluorescence. The proposed chemodosimeter displays the selective detection of Cu 2+ ion and has been effectively utilised for live cell imaging of Cu 2+ in HeLa cells. Graphical abstract: Image 1 Highlights: Fluorescein –NBD Dual fluorophores enabled Chemodosimeter. Turn-on fluorescence via ring opening of spirolactam. Cu(II) induced irreversible hydrolysis. Live cell Imaging in HeLa cells.