Reproducibility of essential elements chromium, manganese, iron, zinc and selenium in spot samples, first-morning voids and 24-h collections from healthy adult men. Issue 3 (24th July 2019)

• Record Type: Journal Article
• Authors: Chen, Heng-Gui; Chen, Ying-Jun; Chen, Chen; Tu, Zhou-Zheng; Lu, Qi; Wu, Ping; Chen, Jun-Xiang; Wang, Yi-Xin; Pan, An
• Is Part Of: British journal of nutrition. Volume 122:Issue 3(2019)
• Page Start: 343
• Page End: 351
• Publication Date: 2019-07-24
• Subjects: Essential elements, -- Urine samples, -- Reproducibility, -- Variability, -- Misclassification; Nutrition -- Periodicals; 572.4
• DOI: 10.1017/S0007114519001193
• Languages: English
• ISSNs: 0007-1145

Abstract

Abstract: Evaluation of Cr, Mn, Fe, Zn and Se in humans is challenged by the potentially high within-individual variability of these elements in biological specimens, which are poorly characterised. This study aimed to evaluate their within-day, between-day and between-month variability in spot samples, first-morning voids and 24-h collections. A total of 529 spot urine samples (including eighty-eight first-morning voids and 24-h collections) were collected from eleven Chinese adult men on days 0, 1, 2, 3, 4, 30, 60 and 90 and analysed for these five elements using inductively coupled plasma-MS. Intraclass correlation coefficients (ICC) were utilised to characterise the reproducibility, and their sensitivity and specificity were analysed to assess how well a single measurement classified individuals' 3-month average exposures. Serial measurements of Zn in spot samples exhibited fair to good reproducibility (creatinine-adjusted ICC = 0·47) over five consecutive days, which became poor when the samples were gathered months apart (creatinine-adjusted ICC = 0·33). The reproducibility of Cr, Mn, Fe and Se in spot samples was poor over periods ranging from days to months (creatinine-adjusted ICC = 0·01–0·12). Two spot samples were sufficient for classifying 60 % of the men who truly had the highest (top 33 %) 3-month average Zn concentrations; for Cr, Mn, Fe and Se, however, at least three specimens were required to achieve similar sensitivities. In conclusion, urinary Cr, Mn, Fe, Zn and Se concentrations showed a strong within-individual variability, and a single measurement is not enough to efficiently characterise individuals' long-term exposures.