A Fully Automated Continuous‐Flow Platform for Fluorescence Quenching Studies and Stern–Volmer Analysis. (27th July 2018)

• Record Type: Journal Article
• Authors: Kuijpers, Koen P. L.; Bottecchia, Cecilia; Cambié, Dario; Drummen, Koen; König, Niels J.; Noël, Timothy
• Is Part Of: Angewandte Chemie. Volume 130:Number 35(2018)
• Page Start: 11448
• Page End: 11452
• Publication Date: 2018-07-27
• Subjects: Automatisierung -- Fluoreszenz-Quenching-Experimente -- Kontinuierlicher Fluss -- Photoredoxkatalyse -- Stern-Volmer; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201805632
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: Herein, we report the first fully automated continuous‐flow platform for fluorescence quenching studies and Stern–Volmer analysis. All the components of the platform were automated and controlled by a self‐written Python script. A user‐friendly software allows even inexperienced operators to perform automated screening of novel quenchers or Stern–Volmer analysis, thus accelerating and facilitating both reaction discovery and mechanistic studies. The operational simplicity of our system affords a time and labor reduction over batch methods while increasing the accuracy and reproducibility of the data produced. Finally, the applicability of our platform is elucidated through relevant case studies.