Rapid Soft Tissue Approximation and Repair Using Laser‐Activated Silk Nanosealants. (5th September 2018)

• Record Type: Journal Article
• Authors: Urie, Russell; Guo, Chengchen; Ghosh, Deepanjan; Thelakkaden, Mitzi; Wong, Valerie; Lee, Jung Keun; Kilbourne, Jacquelyn; Yarger, Jeffery; Rege, Kaushal
• Is Part Of: Advanced functional materials. Volume 28:Number 42(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-09-05
• Subjects: gold nanorods -- sealant -- tissue repair -- tissue welding -- wound healing; Materials -- Periodicals; Chemical vapor deposition -- Periodicals; 620.11
• DOI: 10.1002/adfm.201802874
• Languages: English
• ISSNs: 1616-301X

Abstract

Abstract: Tissue approximation and repair are conventionally performed with sutures and staples, but these means are inherently traumatic. Tissue approximation using laser‐responsive nanomaterials can lead to rapid tissue sealing and repair, and is an attractive alternative to existing clinical methods. Here, the use of laser‐activated nanosealants (LANS) with gold nanorods (GNRs) embedded in silk fibroin polypeptide matrices is demonstrated. The adaptability of LANS for sealing soft tissues is demonstrated using two different modalities: insoluble thin films for internal, intestinal tissue repair, and semisoluble pastes for external repair, shown by skin repair in live mice. Laser repaired intestinal tissue held over seven times more fluid pressure than sutured intestine and also prevented bacterial leakage. Skin incisions in mice closed using LANS' showed indication of increased mechanical strength and faster repair compared to suturing. Laser‐activated silk‐GNR nanosealants rapidly seal soft‐tissue tears and show high promise for tissue approximation and repair in trauma and routine surgery. Abstract : The generation and characterization of silk nanosealants are described for rapid repair of soft tissues following laser irradiation. Water‐resistant silk‐gold nanorod (GNR) nanoselanats were used to seal wet porcine tissues ex vivo, and soluble silk‐GNR sealants were used to seal dry murine skin in vivo. Laser‐activated nanosealants demonstrate higher recovery of tissue mechanical properties compared to conventional sutures and glues.