The macro domain as fusion tag for carrier‐driven crystallization. (2nd November 2016)

• Record Type: Journal Article
• Authors: Wild, Rebekka; Hothorn, Michael
• Is Part Of: Protein science. Volume 26:Number 2(2017)
• Page Start: 365
• Page End: 374
• Publication Date: 2016-11-02
• Subjects: carrier‐driven crystallization -- crystallization tag -- macro domain -- histone macroH2A -- recombinant protein expression; Proteins -- Periodicals; 572.6
• DOI: 10.1002/pro.3073
• Languages: English
• ISSNs: 0961-8368

Abstract

Abstract: Obtaining well‐ordered crystals remains a significant challenge in protein X‐ray crystallography. Carrier‐driven crystallization can facilitate crystal formation and structure solution of difficult target proteins. We obtained crystals of the small and highly flexible SPX domain from the yeast vacuolar transporter chaperone 4 (Vtc4) when fused to a C‐terminal, non‐cleavable macro tag derived from human histone macroH2A1.1. Initial crystals diffracted to 3.3 Å resolution. Reductive protein methylation of the fusion protein yielded a new crystal form diffracting to 2.1 Å. The structures were solved by molecular replacement, using isolated macro domain structures as search models. Our findings suggest that macro domain tags can be employed in recombinant protein expression in E. coli, and in carrier‐driven crystallization. Abstract : PDB Code(s):5LNC