Modulation of hemoglobin dynamics by an allosteric effector. (14th February 2017)

• Record Type: Journal Article
• Authors: Lal, Jyotsana; Maccarini, Marco; Fouquet, Peter; Ho, Nancy T.; Ho, Chien; Makowski, Lee
• Is Part Of: Protein science. Volume 26:Number 3(2017)
• Page Start: 505
• Page End: 514
• Publication Date: 2017-02-14
• Subjects: hemoglobin -- protein dynamics -- neutron spin echo -- X‐ray solution scattering; Proteins -- Periodicals; 572.6
• DOI: 10.1002/pro.3099
• Languages: English
• ISSNs: 0961-8368

Abstract

Abstract: Hemoglobin (Hb) is an extensively studied paradigm of proteins that alter their function in response to allosteric effectors. Models of its action have been used as prototypes for structure‐function relationships in many proteins, and models for the molecular basis of its function have been deeply studied and extensively argued. Recent reports suggest that dynamics may play an important role in its function. Relatively little is known about the slow, correlated motions of hemoglobin subunits in various structural states because experimental and computational strategies for their characterization are challenging. Allosteric effectors such as inositol hexaphosphate (IHP) bind to both deoxy‐Hb and HbCO, albeit at different sites, leading to a lowered oxygen affinity. The manner in which these effectors impact oxygen binding is unclear and may involve changes in structure, dynamics or both. Here we use neutron spin echo measurements accompanied by wide‐angle X‐ray scattering to show that binding of IHP to HbCO results in an increase in the rate of coordinated motions of Hb subunits relative to one another with little if any change in large scale structure. This increase of large‐scale dynamics seems to be coupled with a decrease in the average magnitude of higher frequency modes of individual residues. These observations indicate that enhanced dynamic motions contribute to the functional changes induced by IHP and suggest that they may be responsible for the lowered oxygen affinity triggered by these effectors.