A Self‐Digitization Dielectrophoretic (SD‐DEP) Chip for High‐Efficiency Single‐Cell Capture, On‐Demand Compartmentalization, and Downstream Nucleic Acid Analysis. Issue 35 (27th July 2018)

• Record Type: Journal Article
• Authors: Qin, Yuling; Wu, Li; Schneider, Thomas; Yen, Gloria S.; Wang, Jiasi; Xu, Shihan; Li, Min; Paguirigan, Amy L.; Smith, Jordan L.; Radich, Jerald P.; Anand, Robbyn K.; Chiu, Daniel T.
• Is Part Of: Angewandte Chemie international edition. Volume 57:Issue 35(2018)
• Page Start: 11378
• Page End: 11383
• Publication Date: 2018-07-27
• Subjects: dielectrophoresis -- loop-mediated isothermal amplification -- microfluidic devices -- self-digitization -- single-cell analysis; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201807314
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: The design and fabrication of a self‐digitization dielectrophoretic (SD‐DEP) chip with simple components for single‐cell manipulation and downstream nucleic acid analysis is presented. The device employed the traditional DEP and insulator DEP to create the local electric field that is tailored to approximately the size of single cells, enabling highly efficient single‐cell capture. The multistep procedures of cell manipulation, compartmentalization, lysis, and analysis were performed in the integrated microdevice, consuming minimal reagents, minimizing contamination, decreasing lysate dilution, and increasing assay sensitivity. The platform developed here could be a promising and powerful tool in single‐cell research for precise medicine. Abstract : A self‐digitization dielectrophoretic (SD‐DEP) chip was designed by employing the traditional DEP and insulator DEP to create the local electric field for single‐cell manipulation and downstream nucleic acid analysis. The multistep procedures of cell manipulation, lysis, and chemical analysis were performed in the integrated microdevice, consuming minimal reagents, minimizing contamination, and increasing assay sensitivity.