Enrichment of sulfate reducing anaerobic methane oxidizing community dominated by ANME-1 from Ginsburg Mud Volcano (Gulf of Cadiz) sediment in a biotrickling filter. (July 2018)

• Record Type: Journal Article
• Authors: Bhattarai, Susma; Cassarini, Chiara; Rene, Eldon R.; Zhang, Yu; Esposito, Giovanni; Lens, Piet N.L.
• Is Part Of: Bioresource technology. Volume 259(2018)
• Page Start: 433
• Page End: 441
• Publication Date: 2018-07
• Subjects: Anaerobic oxidation of methane -- Sulfate reduction -- Anaerobic methanotrophs -- Sulfate reducing bacteria -- Biotrickling filter; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2018.03.018
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: AOM coupled to sulfate reduction was demonstrated in the BTF. ANME clades enriched to more than 50% of the archaeal sequences in the BTF. Desulfobacteraceae was increased from 0.2% to 1.8% after 248 days. Abundant anaerobic sulfur oxidizers in the BTF suggested the sulfur cycling. ANME-1 proliferated in the BTF by maintaining high mass transfer and low shear force. Abstract: This study was performed to enrich anaerobic methane-oxidizing archaea (ANME) present in sediment from the Ginsburg Mud Volcano (Gulf of Cadiz) in a polyurethane foam packed biotrickling filter (BTF). The BTF was operated at 20 (±2) °C, ambient pressure with continuous supply of methane for 248 days. Sulfate reduction with simultaneous sulfide production (accumulating ∼7 mM) after 200 days of BTF operation evidenced anaerobic oxidation of methane (AOM) coupled to sulfate reduction. High-throughput sequence analysis of 16S rRNA genes showed that after 248 days of BTF operation, the ANME clades enriched to more than 50% of the archaeal sequences, including ANME-1b (40.3%) and ANME-2 (10.0%). Enrichment of the AOM community was beneficial to Desulfobacteraceae, which increased from 0.2% to 1.8%. Both the inoculum and the BTF enrichment contained large populations of anaerobic sulfur oxidizing bacteria, suggesting extensive sulfur cycling in the BTF.