A NIR fluorescent probe for the rapid detection of Hg2+ in living cells and invivo mice imaging. (11th May 2017)

• Record Type: Journal Article
• Authors: Wang, Jiaoliang; Long, Liping; Xia, Li; Fang, Fang
• Is Part Of: Methods and applications in fluorescence. Volume 5:Number 2(2017:Jun.)
• Publication Date: 2017-05-11
• Subjects: Changsha dye -- near-infrared fluorescent probe -- mercury ion -- mice imaging; Fluorescence spectroscopy -- Periodicals; Fluorescence -- Periodicals; Spectrometry, Fluorescence -- Periodicals; Fluorescence -- Periodicals; Fluorescence; Fluorescence spectroscopy; Periodicals; 535.352
• DOI: 10.1088/2050-6120/aa6d8d
• Languages: English
• ISSNs: 2050-6120

Abstract

Abstract: A near-infrared fluorescent probeNIR-Hg, for the detection of Hg 2+ ion, has been synthesized directly by condensing Changsha dye with 4-Phenyl-3-thiosemicarbazide and the structure has fully characterized by 1 HNMR, 13 CNMR, and ESI-MS. The probe has been designed on the basis of the reaction that Hg 2+ ion promotes thiosemicarbazide to oxazole in aqueous media and had been induced to produce turn-on fluorescence via an irreversible spirolactam ring-opening process. The probeNIR-Hg has exhibited fast response (1 min), high sensitivity with 44-fold fluorescence intensity enhancement under six equivalent amounts of Hg 2+ added, high selectivity over other related metal ions and a low detection limit of 5.8 × 10 −8 M in the phosphate buffer. The linear response range covers the concentration of Hg 2+ from 5 × 10 −7 to 5 × 10 −6 M. In addition, the probe has good cell-membrane permeability, which is suitable for fluorescence imaging for Hg 2+ in living cells and in vivo mice.