Comparison of six digestion methods on fluorescent intensity and morphology of the fluorescent polystyrene beads. (June 2018)

• Record Type: Journal Article
• Authors: Li, Fengmin; Li, Fuyun; Hou, Xiaodong; Luo, Xianxiang; Tu, Haifeng; Zou, Yadan; Sun, Cuizhu; Shi, Mei; Zheng, Hao
• Is Part Of: Marine pollution bulletin. Volume 131(2018)Part A
• Page Start: 515
• Page End: 524
• Publication Date: 2018-06
• Subjects: Fluorescent PS beads -- Digestion -- Fluorescence intensity -- Morphology -- Extraction efficiency; Marine pollution -- Periodicals; Marine Biology -- Periodicals; Water Pollution -- Periodicals; Mer -- Pollution -- Périodiques; Publications périodiques; Pollution des mers; Lutte antipollution; Electronic journals; 363.7394
• DOI: 10.1016/j.marpolbul.2018.04.056
• Languages: English
• ISSNs: 0025-326X

Abstract

Abstract: Effect of digestion methods on fluorescence intensity of fluorescent polystyrene (PS) beads was poorly understood, which may affect the accuracy of toxicity test of the fluorescent PS beads exposed to marine organisms. Therefore, six digestion approaches were compared on fluorescence intensities and properties of three commercial fluorescent PS beads. Among all the protocols, the digestion using KOH (10% w/v, 60 °C) (KOH-digestion) had no effect on the fluorescence intensity, morphology and composition of the three fluorescent PS beads. Moreover, the extraction efficiency ≥ 95.3 ± 0.2% of fluorescent PS beads in Daphnia magna and zebrafish, confirming its feasibility in fluorescent PS beads quantitative analysis. However, the fluorescence intensities of fluorescent PS beads digested by other five protocols were significantly decreased, as well as the change of morphology and composition on fluorescent PS beads. Overall, the KOH-digestion is an optimal protocol for extracting fluorescent PS beads in biological samples. Graphical abstract: Highlights: Fluorescence intensity of fluorescent PS beads was compared by six digestion methods. Morphology and composition of fluorescent PS beads were depended on digestion protocols. KOH-digestion had no effect on fluorescence intensity and morphology of fluorescent PS beads. KOH-digestion showed good performance for extracting fluorescent PS beads from biological samples.