Short-term exercise training reduces anti-inflammatory action of interleukin-10 in adults with obesity. (November 2018)

• Record Type: Journal Article
• Authors: Barry, Julianne C.; Simtchouk, Svetlana; Durrer, Cody; Jung, Mary E.; Mui, Alice L.; Little, Jonathan P.
• Is Part Of: Cytokine. Volume 111(2018)
• Page Start: 460
• Page End: 469
• Publication Date: 2018-11
• Subjects: Interleukin-10 -- Interleukin-6 -- Obesity -- High-intensity interval training -- Moderate-intensity continuous training; Cytokines -- Periodicals; 571.844
• DOI: 10.1016/j.cyto.2018.05.035
• Languages: English
• ISSNs: 1043-4666

Abstract

Highlights: IL10 and IL6 have anti-inflammatory effects in whole-blood cultures. The anti-inflammatory action of IL10 was more potent than IL6. MICT and to a greater extent HIIT reduced anti-inflammatory IL10 action. HIIT and MICT differentially affected IL6 function. Short-term exercise training may decrease anti-inflammatory cytokine effectiveness. Abstract: A key pathological component of obesity is chronic low-grade inflammation, which is propagated by infiltration of immune cells into tissues and overproduction of pro-inflammatory cytokines. Cytokines that possess anti-inflammatory properties, such as interleukin (IL)-10 and IL6, may also play an important role. This study was designed to determine the impact of short-term exercise on the anti-inflammatory action of IL10 and IL6. Thirty-three inactive obese adults were randomized to two weeks of high-intensity interval training (HIIT) or moderate-intensity continuous training (MICT). Fasting blood samples were collected before and after training. Lipopolysaccharide (LPS)-induced tumor necrosis factor (TNF)-α production was measured in whole blood cultures in the presence or absence of IL10 or IL6. IL10 and IL6 receptor expression were measured on circulating monocytes, neutrophils, and T cells. HIIT and MICT reduced the ability of IL10 to inhibit LPS-induced TNFα production, with a greater effect with HIIT (Group × Time and IL10 × Time interactions, p's < 0.05). This reduction in IL10 function was not explained by altered IL10R1 expression, which was unchanged after training (p > 0.05). HIIT and MICT differentially affected IL6 function (Group × Time and IL6 × Time interactions, p's < 0.05) with evidence of reductions in the anti-inflammatory ability of IL6 with HIIT. Neither HIIT nor MICT altered levels of circulating IL10, IL6, or TNFα. The impact of short-term HIIT and MICT resulted in differential effects on anti-inflammatory cytokine function. The clinical implications remain to be determined but these novel findings indicate that measuring anti-inflammatory cytokine action could reveal important immunomodulatory effects of exercise.