Local and Global Dynamics in Intrinsically Disordered Synuclein. Issue 46 (18th October 2018)

• Record Type: Journal Article
• Authors: Rezaei‐Ghaleh, Nasrollah; Parigi, Giacomo; Soranno, Andrea; Holla, Andrea; Becker, Stefan; Schuler, Benjamin; Luchinat, Claudio; Zweckstetter, Markus
• Is Part Of: Angewandte Chemie international edition. Volume 57:Issue 46(2018)
• Page Start: 15262
• Page End: 15266
• Publication Date: 2018-10-18
• Subjects: intrinsically disordered proteins -- NMR spectroscopy -- protein dynamics; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201808172
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Intrinsically disordered proteins (IDPs) experience a diverse spectrum of motions that are difficult to characterize with a single experimental technique. Herein we combine high‐ and low‐field nuclear spin relaxation, nanosecond fluorescence correlation spectroscopy (nsFCS), and long molecular dynamics simulations of alpha‐synuclein, an IDP involved in Parkinson disease, to obtain a comprehensive picture of its conformational dynamics. The combined analysis shows that fast motions below 2 ns caused by local dihedral angle fluctuations and conformational sampling within and between Ramachandran substates decorrelate most of the backbone N−H orientational memory. However, slow motions with correlation times of up to ca. 13 ns from segmental dynamics are present throughout the alpha‐synuclein chain, in particular in its C‐terminal domain, and global chain reconfiguration occurs on a timescale of ca. 60 ns. Our study demonstrates a powerful strategy to determine residue‐specific protein dynamics in IDPs at different time and length scales. Abstract : Three techniques combined : Capturing local and global dynamics from one to hundreds of nanoseconds in the intrinsically disordered protein (IDP) α‐synuclein: the combination of three experimental techniques, which are sensitive to motions on different length and timescales, with long molecular dynamics simulations is a powerful strategy to determine residue‐specific protein dynamics in IDPs.