The blocking of kappa‐opioid receptor reverses the changes in dorsolateral striatum dopamine dynamics during the amphetamine sensitization. Issue 3 (3rd December 2018)

• Record Type: Journal Article
• Authors: Azocar, Victor Hugo; Sepúlveda, Gladys; Ruiz, Catalina; Aguilera, Consuelo; Andrés, Maria Estela; Fuentealba, José Antonio
• Is Part Of: Journal of neurochemistry. Volume 148:Issue 3(2019)
• Page Start: 348
• Page End: 358
• Publication Date: 2018-12-03
• Subjects: amphetamine sensitization -- dopamine -- dorsolateral striatum -- kappa‐opioid receptor -- no‐net flux microdialysis; Neurochemistry -- Periodicals; 616.8042
• DOI: 10.1111/jnc.14612
• Languages: English
• ISSNs: 0022-3042

Abstract

Abstract: Kappa‐opioid receptors (KOR) control dopamine (DA) levels in the striatum and contribute significantly to the progression of drug addiction. Repeated exposure to psychostimulants has been associated with up‐regulated KOR activity and increased DA levels in dorsal striatum. However, it has not been tested if both processes are linked. In this work, we studied if a mechanism mediated by KOR is contributing to the increase in DA levels in the dorsolateral striatum (DLS) after amphetamine (AMPH) sensitization. The AMPH sensitization was assessed after single or repeated once‐a‐day AMPH injections (1 mg/kg). Only repeated AMPH exposure produced a significant locomotor sensitization. No‐net flux microdialysis was used to assess basal DA dialysate, DA extracellular concentration ( C ext ), and DA uptake in DLS of anesthetized rats. The role of KOR on DA dynamics in DLS was evaluated by local perfusion (250 μM) and systemic administration (10 mg/kg) of the KOR antagonist nor‐binaltorphimine. A significant decrease in DA C ext is observed in the DLS after an AMPH challenge in rats exposed to a single dose of AMPH. The decrease in DA C ext was associated with both a decreased basal DA dialysate and an increased DA uptake. Conversely, the expression of AMPH sensitization was accompanied by a significant increase in DA C ext associated with an increased basal DA dialysate and an attenuation in DA uptake. Both local and systemic administration of nor‐binaltorphimine reversed changes in DLS after AMPH pre‐treatment. These findings indicate that endogenous KOR system tunes DLS DA dynamics during the progression to AMPH sensitization. Abstract : We proposed that dynorphin contributes to dopamine (DA) dynamics during progression to amphetamine (AMPH) sensitization modifying DA transporter activity. A significant decrease in DA extracellular levels is observed in the dorsolateral striatum (DLS) after a single dose of AMPH. The decrease in DA extracellular is associated with an increased DA uptake. Conversely, the expression of AMPH sensitization is accompanied by a significant increase in DA extracellular levels associated with attenuation in DA uptake. The administration of nor‐BNI reverses the changes in DLS after AMPH pre‐treatment tending to normalize DA extracellular levels modifying DA uptake activity.